The US team of, , and Phelps won at the in a time of 3:29.34 and in doing so broke the world record by 1.34 seconds.
The defending Olympic champions and world record holders led from start to finish.
(L-R) Hansen, Phelps, Peirsol and Lezak hold the American flag (Photo credit: Al Bello/Getty Images)
Peirsol gave them a great start with a 53.16sec split. Hansen increased the lead on the world record split taking it 0.39 seconds under at the race's halfway mark. Brenton Rickard swam his breaststroke leg in 58.56, faster than Hansen, to bring thens into second place.
Phelps fought off the Australian challenge in the Butterfly leg before Lezak kepton his shoulder until the wall.
The Australian team of Hayden Stoeckel, Andrew Lauterstein, Rickard and Sullivan finished 0.70 seconds behind the US to win silver in an Oceania record 3:30.04, also under the old world record.
Australia went into the race as world champions but only as beneficiaries of a US disqualification at the 2007 world championships.
Japan's team of Miyashita Junichi, Kitajima Kosuke, Fujii Takuro and Sato Hisayoshi took bronze in an Asian record 3:31.18.
Russia finished fourth in a European record 3:31.92.